In our recent paper by in collaboration with the group of Dr. Nicolás Unsain (INIMEC, Córdoba), we present a protocol to perform expansion fluorescence microscopy quantitatively. We validate the protocol comparing the expansion microscopy images to images obtain with STED, of the membrane-associated periodic skeleton of neurons.

Gaby F. Martínez et al. “Quantitative expansion microscopy for the characterization of the spectrin periodic skeleton of axons using fluorescence microscopy”

Scientific Reports 10 (2020) 2917 pdf