In our recent paper by in collaboration with the group of Dr. Nicolás Unsain (INIMEC, Córdoba), we present a protocol to perform expansion fluorescence microscopy quantitatively. We validate the protocol comparing the expansion microscopy images to images obtain with STED, of the membrane-associated periodic skeleton of neurons.

Gaby F. Martínez et al. “Quantitative expansion microscopy for the characterization of the spectrin periodic skeleton of axons using fluorescence microscopy”